Recombinant Rat TNFR1/TNFRSF1A Protein (Fc Tag)

Specifications

Catalog number

PKSR030339

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD) . CD120a can activate the transcription factor NF-κ B, mediate apoptosis, and regulate inflammation processes.

Synonyms

TNFRSF1A; Tnfr-1; Tnfr1

UniProt

P22934

Accession Number

P22934

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met1-Ala211

Applications

Cell culture; ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα. 2. Immobilized mouse TNFa (80-235) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.9 kDa

Observed Molecular Weight

59 kDa

Species

Rat