Recombinant Rat IL1R1/CD121a Protein (His & Fc Tag)

Specifications

Catalog number

PKSR030395

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA) . IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

Abbreviation

IL1R1/CD121a

Synonyms

CD121 antigen-like family member A; CD121A; D2S1473; IL-1 RI; IL-1R-1; IL-1R-alpha; IL-1R1; IL-1RT-1; IL-1RT1; IL1R; IL1RA; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; P80

UniProt

A6INN1

Accession Number

NP_037255.3

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Lys 352

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit IL1α-dependent proliferation in D10.G4. 1 mouse helper T cells (Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds) : IRL Press. 272.) . The ED50 for this effect is typically 5-20 μg/ml in the presence of 40 pg/mL of recombinant human IL1a.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

64.6 kDa

Observed Molecular Weight

90-95 kDa

Species

Rat