Recombinant SARS-CoV-2 Methyltransferase / ME Protein (His Tag)

Specifications

Catalog number

PKSR030513

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronavirus encodes the 2'-O-MTase (2'O Methyltransferase) that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting on the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral durgs development which can against SARS-CoV-2, SARS-CoV, MERS-CoV or other RNA and DNA viruses.

Synonyms

ME; Methyltransferase

Accession Number

YP_009724390.1

Expression System

E.coli

Tag

C-His

Sequence

Ser6799-Asn7096

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.5 kDa

Species

SARS-CoV-2