Recombinant Swine TNF alpha protein (His Tag)

Specifications

Catalog number

PKSS000010-01

Size / count

5 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1, p55R) and TNF-R2 (TNF receptor type 2, p75R) . TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

Abbreviation

TNF alpha

Synonyms

Cachectin; Cytotoxin; Differentiation-inducing factor (DIF) ; Necrosin; TNFSF2; TNS F1A

UniProt

P23563

Accession Number

P23563

Expression System

E.coli

Tag

C-His

Sequence

Leu 77-Leu 232

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.1 kDa

Observed Molecular Weight

19 kDa

Species

Porcine