Recombinant SARS-CoV S1 Protein (His Tag) (Active)

Specifications

Catalog number

PKSV030101

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Synonyms

SARS; coronavirus s1 Protein; coronavirus s2 Protein; coronavirus spike Protein; cov spike Protein; ncov RBD Protein; ncov s1 Protein; ncov s2 Protein; ncov spike Protein; novel coronavirus RBD Protein; novel coronavirus s1 P

Accession Number

AAX16192.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met1-Arg667

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human ACE2 protein (Fc tag) (PKSH031870) at 2μg/mL (100μL/well) can bind Recombinant SARS-CoV S1 Protein (His Tag) (Active) (PKSV030101), the EC50 of SARS-CoV S1 Protein (His Tag) (Active) (PKSV030101) is 400-850 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

74.4 kDa

Observed Molecular Weight

85.8 kDa

Species

SARS