Recombinant HCV (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag)

Specifications

Catalog number

PKSV030179

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity) . Cleaves and inhibits the host antiviral protein MAVS.

Synonyms

HCV; NS3 Protein

Accession Number

NP_803144.1

Expression System

E.coli

Tag

N-GST

Sequence

Thr1356-Thr1459

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.9 kDa

Species

HCV