Recombinant MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)

Specifications

Catalog number

PKSV030241

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Synonyms

MERS-CoV; RBD Protein; S Pr; coronavirus s1 Protein; coronavirus s2 Protein; coronavirus spike Protein; cov spike Protein; ncov RBD Protein; ncov s1 Protein; ncov s2 Protein; ncov spike Protein

Accession Number

AFS88936.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Glu725

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized Spike Protein S1 (aa 1-725) (Cat: PKSV030241) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4 (Cat: PKSH033811) . The EC50 of of biotinylated DPP4 (Cat: PKSH033811) is 0.6-1. 39 μg/ml. 2. Immobilized Spike Protein S1 (aa 1-725) (Cat: PKSV030241) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: PKSH030456) . The EC50 of biotinylated Fc-DPP4 (Cat: PKSH030456) is 0.02-0.05 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

79.9 kDa

Observed Molecular Weight

94 kDa

Species

MERS-CoV