Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)

Specifications

Catalog number

PKSV030264

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

Synonyms

E protein; Zika virus; strain Zika SPH2015

Accession Number

ALU33341.1

Expression System

E.coli

Tag

N-His-MBP

Sequence

Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592

Endotoxin

Please contact us for more information.

Purity

> 75 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.2 kDa

Species

ZIKV