Recombinant HIV p24 protein (C-6His)

Specifications

Catalog number

PKSV030277

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Abbreviation

P24

Synonyms

Capsid protein p24; Human immunodeficiency virus 1; Human immunodeficiency virus type 1 p24

UniProt

P12493

Accession Number

P12493

Expression System

E.coli

Tag

C-His

Sequence

Met142-Gly355

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

24.6 kDa

Observed Molecular Weight

25 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Trehalose, 5% Mannitol,0.01% Tween 80, 1mM EDTA, pH 9.0.

Species

HIV