Recombinant SARS-CoV Nucleocapsid Protein

Specifications

Catalog number

PKSV030290-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Abbreviation

Nucleocapsid

Synonyms

SARS-CoV coronavirus NP Protein; SARS-CoV novel coronavirus Nucleoprotein; SARS-CoV np Protein

UniProt

P59595

Accession Number

P59595

Expression System

E.coli

Tag

N-His

Sequence

Met1-Ala422

Endotoxin

Please contact us for more information.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

49.7 kDa

Observed Molecular Weight

58 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5

Species

SARS