Recombinant SARS-CoV-2 Nucleocapsid Protein

Specifications

Catalog number

PKSV030309-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Synonyms

SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 novel coronavirus Nucleoprotein Protein; SARS-CoV-2 np Protein

UniProt

P0DTC2

Accession Number

QHD43423.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Ala419

Applications

ELISA

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized 2019-nCoV NP Antibody (6G9) -Fc at 5μg/ml (100 μl/well) can bind Recombinant 2019-nCoV Nucleocapsid Protein (Mammalian) -His. The ED50 of 2019-nCoV Nucleocapsid Protein-His is 0.172 ug/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.4 kDa

Observed Molecular Weight

50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5

Species

SARS-CoV-2