Recombinant SARS-CoV-2 N Protein biotinylated (N-6His-Avi)

Specifications

Catalog number

PKSV030311-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Synonyms

SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 novel coronavirus; SARS-CoV-2 np Protein

UniProt

P0DTC2

Accession Number

QHD43423.2

Expression System

E.coli

Conjugation

Biotinylated

Tag

N-His-Avi

Sequence

Met1-Ala419

Applications

ELISA

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized 2019-nCoV NP Antibody (6G9) at 5μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His-Avi (PKSV030311) . The ED50 of PKSV030311 is 92 ng/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

51.3 kDa

Observed Molecular Weight

55-60 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH 7.5

Species

SARS-CoV-2