Recombinant SARS-CoV-2 NSP12 protein

Specifications

Catalog number

PKSV030327-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The RdRp of SARS-CoV-2 is composed of a catalytic subunit known as nsp12 as well as two accessory subunits, nsp8 and nsp7. The structure of this RdRp has recently been reported; it is highly similar to the RdRp of SARS-CoV, a zoonotic coronavirus that spread into the human population in 2002. The nsp12 subunit contains an N-terminal nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain, an interface domain and a C-terminal RdRp domain. The RdRp domain resembles a right hand, comprising the fingers, palm and thumb subdomains that are found in all single-subunit polymerases. Subunits nsp7 and nsp8 bind to the thumb, and an additional copy of nsp8 binds to the fingers domain. Structural information is also available for nsp8–nsp7 complexes.

Synonyms

RNA-directed RNA polymerase; RdRp; SARS-CoV 2 nsp12

UniProt

P0DTC2

Accession Number

QHD43415.1

Expression System

E. coli

Tag

N-Gst

Sequence

Asp4891-Val5212

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

64.0 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.

Species

SARS-CoV-2