Recombinant SARS-CoV-2 N Protein (R203K, G204R) (His Tag)

Specifications

Catalog number

PKSV030341

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Synonyms

SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 novel coronavirus; SARS-CoV-2 np Protein

Accession Number

YP_009724397.2

Expression System

E.coli

Tag

N-His

Sequence

Ser2-Ala419 (R203K, G204R)

Endotoxin

Please contact us for more information.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.5 kDa

Species

SARS-CoV-2