Recombinant SARS-CoV-2 Spike RBD-SD1 (V367F) (His Tag)

Specifications

Catalog number

PKSV030386

Size / count

500 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Synonyms

2019-nCoV S protein RBD; S protein RBD; SARS-CoV-2 Spike RBD

UniProt

P0DTC2

Accession Number

QHD43416.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Arg319-Ser591 (Val367Phe)

Applications

ELISA

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

PKSR030477 Immobilized Human ACE-2-Fc (PKSR030492) at 5μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-His (PKSR030477) . The ED50 of PKSR030477 is 1.7ug/ml. PKSV030386 Immobilized Human ACE-2-Fc (PKSR030492) at 5μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1 (V367F, C-6His) (PKSV030386) . The ED50 of PKSV030386 is 0.8ug/ml. , PKSR030477 Immobilized Anti-2019-nCoV S1 mAb (5D9) at 5μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-His (PKSR030477) . The ED50 of PKSR030477 is 1. 28 ug/ml. PKSV030386 Immobilized Anti-2019-nCoV S1 mAb (5D9) at 5μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1 (V367F, C-6His) (PKSV030386) . The ED50 of PKSV030386 is 2. 55 ug/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

31.4 kDa

Observed Molecular Weight

30-40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

SARS-CoV-2