Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™

About this product

Biotinylated SARS-CoV-2 S1 protein (D614G), His,Avitag (S1N-C82E3) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1 (D614G)).

Specifications

Catalog number

S1N-C82E3-25ug

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 78.5 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Val 16 - Arg 685

Host

HEK 293

Target

Spike S1

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein (D614G), His,Avitag (Cat. No. S1N-C82E3) with a linear range of 0.02-0.625 μg/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

78.5 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C