SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified)

Catalog number SPN-C52Hp-500ug

Discontinued

Price for SPN-C52Hp-500ug

Size / count 500 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 S protein (N501Y,D614G), His Tag (SPN-C52Hp) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (N501Y, D614G)). The N501Y/D614G mutations were identified in multiple SARS-CoV-2 variants which emerged in the United Kingdom (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01), South Africa(known as B.1.351 or 20C/501Y.V2), and Brazil (Known as P.1). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Specifications

Catalog number: SPN-C52Hp-500ug
Size / count: 500 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 170-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Val 16 - Pro 1213
Host: HEK 293
Target: Spike protein
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike Trimer (N501Y, D614G), His Tag (Cat. No. SPN-C52Hp) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: 0.1 M Sodium citrate, pH5.5
Additives: Trehalose
Molecular Weight: 138.0 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified)?

The catalog number for SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified) is SPN-C52Hp-500ug.

Is SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified) in stock?

SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified)?

SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified) used for?

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.