Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified)

Catalog number SPD-C82Ef-25ug

Discontinued

Price for SPD-C82Ef-25ug

Size / count 25 µg

This variant is discontinued and cannot be added to your cart.

About this product

Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (SPD-C82Ef) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (S477N, E484K)). The mutations (S477N, E484K) were identified in the SARS-CoV-2 variants which emerged in Europe (known as B.1.620).

Specifications

Catalog number: SPD-C82Ef-25ug
Size / count: 25 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Biotin-labeled
Tag: C-10×His & C-Avi
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (Cat. No. SPD-C82Ef) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 28.2 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified)?

The catalog number for Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified) is SPD-C82Ef-25ug.

Is Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified) in stock?

Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified)?

Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified) is manufactured by CRB.

What is the estimated delivery time for Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified)?

Estimated delivery time for Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified) is approximately 10 business day(s), depending on your location.

What is Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified) used for?

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.