Biotinylated SARS-CoV-2 Spike Trimer Protein (C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N), His,Avitag™ (MALS verified)

About this product

Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (SPN-C82Ek) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N were identified in the SARS-CoV-2 variant C.1.2. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Specifications

Catalog number

SPN-C82Eq-200ug

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 139.5 kDa. The protein migrates as 180-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Val 16 - Pro 1213

Host

HEK 293

Target

Spike protein

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (Cat. No. SPN-C82Eq) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

139.5 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C