Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified)

Catalog number SPD-C82En-25ug

Discontinued

Price for SPD-C82En-25ug

Size / count 25 µg

This variant is discontinued and cannot be added to your cart.

About this product

Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag (SPD-C82En) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (Y449H, E484K, N501Y)). The mutations Y449H, E484K, N501Y were identified in the SARS-CoV-2 variant C.1.2.

Specifications

Catalog number: SPD-C82En-25ug
Size / count: 25 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Biotin-labeled
Tag: C-10×His & C-Avi
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag (Cat. No. SPD-C82En) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 28.2 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified)?

The catalog number for Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified) is SPD-C82En-25ug.

Is Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified) in stock?

Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified)?

Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified) is manufactured by CRB.

What is the estimated delivery time for Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified)?

Estimated delivery time for Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified) is approximately 10 business day(s), depending on your location.

What is Biotinylated SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His,Avitag™ (MALS verified) used for?

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.