SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified)

Catalog number SPN-C522c-200ug

Discontinued

Price for SPN-C522c-200ug

Size / count 200 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (SPN-C522c) is expressed from human 293 cells (HEK293). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.3; GISAID clade: GRA; Nextstrain clade: 21M). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Specifications

Catalog number: SPN-C522c-200ug
Size / count: 200 µg
Dry ice: Yes
Hazardous: No
Discontinued: Yes
Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.8 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Host: HEK 293
Target: Spike protein
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C522c) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 2-31 ng/mL (QC tested).
Format: Liquid
Buffer: 0.1 M Sodium citrate, pH5.5
Molecular Weight: 137.8 kDa
Shipping Conditions: Dry ice
Storage Conditions: -70°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified)?

The catalog number for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) is SPN-C522c-200ug.

Is SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) in stock?

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Are there any special shipping requirements for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified)?

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) is requires dry ice shipping.

Who manufactures SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified)?

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike Trimer Protein, His Tag (BA.3/Omicron) (MALS verified) used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.