Biotinylated SARS-CoV-2 Spike NTD Protein, His,Avitag™ (BA.2/Omicron) (MALS verified)

About this product

Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (BA.2/Omicron) (SPD-C82H5) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, G142D, V213G)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L).

Specifications

Catalog number

SPD-C82H5-25ug

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Ser 13 - Leu 303

Host

HEK 293

Target

Spike NTD

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (BA.2/Omicron) (Cat. No. SPD-C82H5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (Cat. No. SPD-M121) with a linear range of 0.1-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.3 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C