SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified)

Catalog number SPN-C522k-10mg

Discontinued

Price for SPN-C522k-10mg

Size / count 10 mg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike Trimer, His Tag (BA.2.75/Omicron) (SPN-C522k) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.75). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Specifications

Catalog number: SPN-C522k-10mg
Size / count: 10 mg
Dry ice: Yes
Hazardous: No
Discontinued: Yes
Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Val 16 - Pro 1213
Host: HEK 293
Target: Spike protein
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2.75/Omicron) (Cat. No. SPN-C522k) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-0.8 ng/mL (QC tested).
Format: Liquid
Buffer: 0.1 M Sodium citrate, pH5.5
Molecular Weight: 138.0 kDa
Shipping Conditions: Dry ice
Storage Conditions: -70°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified)?

The catalog number for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) is SPN-C522k-10mg.

Is SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) in stock?

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Are there any special shipping requirements for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified)?

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) is requires dry ice shipping.

Who manufactures SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified)?

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.75/Omicron) (MALS verified) used for?

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.