Anti-MMP8 Antibody Picoband® (Biotine Conjugate)

About this product

Boster Bio Anti-MMP8 Antibody Picoband® catalog # PB9727. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9727-Biotin

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MMP8 (Matrix metalloproteinase 8) is a member of the family of matrix metalloproteinases. It is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8 is mapped to 11q21-q22. MMP8 is an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The enzyme exhibits 58% homology to human fibroblast collagenase and has the same domain structure. It consists of a 20-residue signal peptide, and an 80-residue propeptide that is lost on autolytic activation by cleavage of an M-L bond. MMP8 was found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Matrix metalloproteinases (MMPs) have fundamental roles in tumor progression, but most clinical trials with MMP inhibitors have not shown improvements in individuals with cancer. MMP8 has a paradoxical protective role in cancer and provides a genetic model to evaluate the molecular basis of gender differences in cancer susceptibility.

Synonyms

Neutrophil collagenase;3.4.24.34;Collagenase 2;Matrix metalloproteinase-8;MMP-8;Mmp8;

Gene Name

Neutrophil collagenase

UniProt

O70138

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse MMP-8, different from the related human sequence by eleven amino acids, and from the related rat sequence by nine amino acids.

Clonality

Polyclonal

Tissue Specificity

Neutrophils. Expressed in uterus. Low levels in kidney and muscle.

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat <br>ELISA, 0.1-0.5μg/ml, -<br>Western blot, 0.1-0.5μg/ml, Mouse, Rat<br>

Subcellular Location

Cytoplasmic granule. Secreted, extracellular space, extracellular matrix. Stored in intracellular granules and released during inflammatory conditions.

Protein Name

Neutrophil collagenase