Anti-SMYD3 Antibody Picoband® (Biotine Conjugate)

About this product

Boster Bio Anti-SMYD3 Antibody Picoband® catalog # PB9893. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9893-Biotin

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene. The International Radiation Hybrid Mapping Consortium mapped the SMYD3 gene to chromosome 1. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Histone-lysine N-methyltransferase SMYD3;2.1.1.43;SET and MYND domain-containing protein 3;Zinc finger MYND domain-containing protein 1;SMYD3;ZMYND1, ZNFN3A1;

Gene Name

Histone-lysine N-methyltransferase SMYD3

UniProt

Q9H7B4

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SMYD3, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Histone methyltransferase. Specifically methylates 'Lys- 4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat <br>Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cytoplasm . Nucleus . Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.

Protein Name

Histone-lysine N-methyltransferase SMYD3