Anti-DFFA/ICAD Antibody Picoband® (Biotine Conjugate)

About this product

Boster Bio Anti-DFFA/ICAD Antibody Picoband® catalog # A03671-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03671-4-Biotin

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Synonyms

Acetyl-CoA carboxylase 2; ACC-beta; Biotin carboxylase; ACACB; ACC2, ACCB

Gene Name

acetyl-CoA carboxylase beta

UniProt

O00273

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DFFA/ICAD recombinant protein (Position: E14-S308).

Clonality

Polyclonal

Tissue Specificity

Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (PubMed:20457939, PubMed:19236960, PubMed:20952656). Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (PubMed:20457939, PubMed:19236960, PubMed:20952656). Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (By similarity). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1 (By similarity).

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Rat<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Nucleus. Cytosol.

Protein Name

Breast cancer metastasis-suppressor 1