Anti-NDUFA1 Antibody Picoband® (Biotine Conjugate)

About this product

Boster Bio Anti-NDUFA1 Antibody catalog # PA1661. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1661-Biotin

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NDUFA1 (NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.

Synonyms

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1;Complex I-MWFE;CI-MWFE;NADH-ubiquinone oxidoreductase MWFE subunit;NDUFA1;

Gene Name

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

UniProt

O15239

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Primarily expressed in heart and skeletal muscle.

Applications

IF,IHC,IHC-F,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human<br>

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.

Sequence Similarities

Contains 1 death domain.

Protein Name

NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1