Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband® (Biotine Conjugate)

About this product

Boster Bio Anti-HSC70 Interacting Protein HIP/ST13 Antibody catalog # PA1935. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1935-Biotin

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ST13 (Suppression of Tumorigenicity 13), also known as P48 or HIP, is a protein that in humans is encoded by the ST13 gene. ST13 is an abundant, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, but is absent from the mature receptor complex. Zhang et al. (1998) mapped the ST13 gene to chromosome 22q13 by fluorescence in situ hybridization. They noted that colorectal, breast, and ovarian carcinomas frequently show loss of heterozygosity at this site. Using a yeast 2-hybrid assay, Hohfeld et al. (1995) showed that rat Hip bound Hsc70 (HSPA8). One Hip oligomer bound the ATPase domains of at least 2 Hsc70 molecules, and binding was dependent on activation of the Hsc70 ATPase by Hsp40 (DNAJB1). Hip stabilized the ADP-bound form of Hsc70, which had a high affinity for a test protein substrate. Hohfeld et al. (1995) concluded that HIP contributes to interactions of HSC70 with target proteins through its own chaperone activity.

Synonyms

Hsc70-interacting protein;Hip;Aging-associated protein 2;Progesterone receptor-associated p48 protein;Protein FAM10A1;Putative tumor suppressor ST13;Renal carcinoma antigen NY-REN-33;Suppression of tumorigenicity 13 protein;ST13;AAG2, FAM10A1, HIP, SNC6;

Gene Name

Hsc70-interacting protein

UniProt

P50502

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ST13, different from the related rat and mouse sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed in different tissues.

Applications

IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity). .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br>

Subcellular Location

Cytoplasm .

Sequence Similarities

Belongs to the FAM10 family.

Protein Name

Hsc70-interacting protein