Anti-ATP5F1A Antibody Picoband®, Cy3 Conjugate

About this product

Boster Bio Anti-ATP5F1A Antibody Picoband® catalog # A32267-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A32267-2-Cy3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP synthase F1 subunit alpha, mitochondrial is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Synonyms

Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29

Gene Name

retrotransposon Gag like 10

Gene ID

498

UniProt

P25705

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ATP5F1A recombinant protein (Position: R12-Q520).

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Could induce apoptosis in a BH3 domain-dependent manner. The interaction network of Bcl-2 family members may play a key role in modulation RTL10/BOP intrinsic apoptotic signaling activity.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Akiyama, S., Endo, H., Inohara, N., Ohta, S., Kagawa, Y. Gene structure and cell type-specific expression of the human ATP synthase alpha subunit. Biochim. Biophys. Acta 1219: 129-140, 1994. 2. Baran, A. A., Silverman, K. A., Zeskand, J., Koratkar, R., Palmer, A., McCullen, K., Curran, W. J., Jr., Edmonston, T. B., Siracusa, L. D., Buchberg, A. M. The modifier of Min 2 (Mom2) locus: embryonic lethality of a mutation in the Atp5a1 gene suggests a novel mechanism of polyp suppression. Genome Res. 17: 566-576, 2007. 3. Godbout, R., Pandita, A., Beatty, B., Bie, W., Squire, J. A. Comparative genomic hybridization analysis of Y79 and FISH mapping indicate the amplified human mitochondrial ATP synthase alpha-subunit gene (ATP5A) maps to chromosome 18q12-q21. Cytogenet. Cell Genet. 77: 253-256, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

14377 MW

Observed Molecular Weight

54 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

Lymphocyte antigen 6A-2/6E-1