Anti-TRMT61B Antibody Picoband®, Cy3 Conjugate

About this product

Boster Bio Anti-TRMT61B Antibody Picoband® catalog # A12682-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12682-2-Cy3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Enables mRNA (adenine-N1-)-methyltransferase activity; rRNA (adenine) methyltransferase activity; and tRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation; mitochondrial tRNA methylation; and protein homooligomerization. Located in mitochondrion.

Synonyms

Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3FJ

Gene Name

histone cluster 1 H3 family member a/b/c/d/e/f/g/h/i/j

Gene ID

55006

UniProt

Q9BVS5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TRMT61B recombinant protein (Position: R7-N477).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Applications

ELISA,Flow Cytometry,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bar-Yaacov, D., Frumkin, I., Yashiro, I., Chujo, T., Ishigami, Y., Chemla, Y., Blumberg, A., Schlesinger, O., Bieri, P., Greber, B., Ban, N., Zarivach, R., Alfonta, L., Pilpel, Y., Suzuki, T., Mishmar, D. Mitochondrial 16S rRNA is methylated by tRNA methyltransferase TRMT61B in all vertebrates. PLoS Biol. 14: e1002557, 2016. Note: Erratum: PLoS Biol. 15: e1002594, 2017. 2. Chujo, T., Suzuki, T. Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs. RNA 18: 2269-2276, 2012. 3. Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2021.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

57 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cytoplasm.