Anti-NDUFAF4 Antibody Picoband®, Cy3 Conjugate

About this product

Boster Bio Anti-NDUFAF4 Antibody Picoband® catalog # A11650-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A11650-1-Cy3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NADH:ubiquinone oxidoreductase complex assembly factor 4, (NDUFAF4) also known as Hormone-regulated proliferation-associated protein of 20 kDa, (HRPAP20) or C6orf66 is a protein that in humans is encoded by the NDUFAF4 gene. NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.

Synonyms

Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24

Gene Name

eva-1 homolog A, regulator of programmed cell death

Gene ID

29078

UniProt

Q9P032

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NDUFAF4 recombinant protein (Position: K24-K175).

Clonality

Polyclonal

Tissue Specificity

Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Baertling, F., Sanchez-Caballero, L., van den Brand, M. A. M., Wintjes, L. T., Brink, M., van den Brandt, F. A., Wilson, C., Rodenburg, R. J. T., Nijtmans, L. G. J. NDUFAF4 variants are associated with Leigh syndrome and cause a specific mitochondrial complex I assembly defect. Europ. J. Hum. Genet. 25: 1273-1277, 2017. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/8/2008. 3. Karp, C. M., Pan, H., Zhang, M., Buckley, D. J., Schuler, L. A., Buckley, A. R. Identification of HRPAP20: a novel phosphoprotein that enhances growth and survival in hormone-responsive tumor cells. Cancer Res. 64: 1016-1025, 2004.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

21 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Endoplasmic reticulum membrane, Single-pass membrane protein, Lysosome membrane.