- Catalog number
- A14219-2-Cy3
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicerindependent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
- Synonyms
- 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
- Gene Name
- Serine/threonine-protein kinase AtPK1/AtPK6
- Gene ID
- 113802
- UniProt
- Q5T8I9
- Host
- Rabbit
- Reactivity
- Human,Mouse
- Cross Reactivity
- No cross reactivity with other proteins.
- Immunogen
- E.coli-derived human HENMT1 recombinant protein (Position: Q45-D388). Human HENMT1 shares 70.3% and 73.3% amino acid (aa) sequence identity with mouse and rat HENMT1, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in all tissues.
- Applications
- WB,ICC,IF,Flow Cytometry,ELISA
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Function
- Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
- Components
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
- References & Citations
- 1. Ameres, S. L., Horwich, M. D., Hung, J.-H., Xu, J., Ghildiyal, M., Weng, Z., Zamore, P. D. Target RNA-ed trimming and tailing of small silencing RNAs. Science 328: 1534-1539, 2010.
2. Gross, M. B. Personal Communication. Baltimore, Md. 5/21/2014.
3. Okamura, K., Chung, W.-J., Ruby, J. G., Guo, H., Bartel, D. P., Lai, E. C. The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature 453: 803-806, 2008.
- Storage Conditions
- At -20℃ for one year from date of receipt.
After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months.
Avoid repeated freezing and thawing.
- Calculated Molecular Weight
- 52588 MW
- Observed Molecular Weight
- 45 kDa
- Fragment
- IgG
- Applications Notes
- Western blot, 0.25-0.5 μg/ml, Human, Mouse<br>
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br>
Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br>
ELISA, 0.1-0.5 μg/ml, -<br>
- Subcellular Location
- Cytoplasm. Nucleus.
- Protein Name
- Serine/threonine-protein kinase AtPK1/AtPK6