Anti-IKK gamma/IKBKG Antibody Picoband®, Cy3 Conjugate

About this product

Boster Bio Anti-IKK gamma/IKBKG Antibody catalog # PA1551. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1551-Cy3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NF-kappa-B essential modulator (NEMO), also known as inhibitor of nuclear factor kappa-B kinase subunit gamma (IKK-γ), is a protein that in humans is encoded by the IKBKG gene. This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Synonyms

NF-kappa-B essential modulator;NEMO;IkB kinase-associated protein 1;IKKAP1;mFIP-3;Inhibitor of nuclear factor kappa-B kinase subunit gamma;I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma;NF-kappa-B essential modifier;Ikbkg;Nemo;

Gene Name

NF-kappa-B essential modulator

UniProt

O88522

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse IKK gamma, different from the relative rat sequence by three amino acids.

Clonality

Polyclonal

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

References & Citations

1. Rothwarf, D. M., Zandi, E., Natoli, G., Karin, M.IKK-gamma is an essential regulatory subunit of the I-kappa-B kinase complex.Nature 395: 297-300, 1998. 2. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S. T., Weil, R., Agou, F., Kirk, H. E., Kay, R. J., Israel, A.Complementation cloning of NEMO, a component of the I-kappa-B kinase complex essential for NF-kappa-B activation.Cell 93: 1231-1240, 1998.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

47972 MW

Observed Molecular Weight

68 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse<br>

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.

Protein Name

NF-kappa-B essential modulator(NEMO)