Anti-Zonula occludens protein 3/TJP3 Antibody Picoband®, Cy3 Conjugate

About this product

Boster Bio Anti-Zonula occludens protein 3/TJP3 Antibody catalog # PA1973. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1973-Cy3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TJP3 (TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al. (1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds ly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al. (2002) determined that Tjp3 interacted with PATJ (INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.

Synonyms

Tight junction protein ZO-3;Tight junction protein 3;Zona occludens protein 3;Zonula occludens protein 3;Tjp3;Zo3;

Gene Name

Tight junction protein ZO-3

UniProt

Q9QXY1

Host

Rabbit

Reactivity

Rat,Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Zonula occludens protein 3, identical to the related rat sequence.

Clonality

Polyclonal

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Haskins, J., Gu, L., Wittchen, E. S., Hibbard, J., Stevenson, B. R. ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. J. Cell Biol. 141: 199-208, 1998. 2. Itoh, M., Furuse, M., Morita, K., Kubota, K., Saitou, M., Tsukita, S. binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J. Cell Biol. 147: 1351-1363, 1999. 3. Roh, M. H., Liu, C.-J., Laurinec, S., Margolis, B. The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions. J. Biol. Chem. 277: 27501-27509, 2002.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

99324 MW

Observed Molecular Weight

150 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat<br>Western blot, 0.1-0.5μg/ml, Rat, Mouse<br>

Subcellular Location

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction .

Sequence Similarities

Belongs to the MAGUK family.

Protein Name

Tight junction protein ZO-3