Anti-Csk Antibody Picoband® (Dylight488 Conjugate)

About this product

Boster Bio Anti-Csk Antibody Picoband® catalog # PB9102. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9102-Dylight488

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CSK also known as C-Src kinase, is a non-receptor protein tyrosine kinase protein that in humans is encoded by the CSK gene. It is mapped to 15q24.1. CSK suppresses activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal site in Src. Csk's control of the Src family activity is widely thought to be central to regulation of the immune response. CSK can downregulate tyrosine kinase activity of the SRC oncoprotein through tyrosine phosphorylation of the SRC carboxy terminus. Since cell transformation by SRC oncoproteins is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene.

Synonyms

Tyrosine-protein kinase CSK;2.7.10.2;C-Src kinase;Protein-tyrosine kinase CYL;CSK;

Gene Name

Tyrosine-protein kinase CSK

UniProt

P41240

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK.

Clonality

Polyclonal

Tissue Specificity

Expressed in lung and macrophages. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br>Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Cytoplasm . Cell membrane . Mainly cytoplasmic, also present in lipid rafts. .

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

Protein Name

Tyrosine-protein kinase CSK