Anti-Calcineurin A/PPP3CA Antibody Picoband® (Dylight488 Conjugate)

About this product

Boster Bio Anti-Calcineurin A/PPP3CA Antibody Picoband® catalog # PB9382. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9382-Dylight488

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Calcineurin A is also known as PPP3CA. It is mapped to 4q24. Semsarian et al. (1999) and Musaro et al. (1999) independently showed that IGF1stimulates skeletal muscle hypertrophy and a switch to glycolytic metabolism by activating calcineurin A and inducing the nuclear translocation of transcription factor NFATC1. Semsarian et al. (1999) found that hypertrophy was suppressed by the calcineurin inhibitors cyclosporin A or FK506, but not by inhibitors of the MAP kinase or phosphatidylinositol-3-OH kinase pathways. Musaro et al. (1999) showed that expression of a dominant-negative calcineurin mutant also repressed myocyte differentiation and hypertrophy. Musaro et al. (1999) demonstrated that either IGF1 or activated calcineurin induces expression of transcription factor GATA2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of NFATC1.

Synonyms

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform;3.1.3.16;CAM-PRP catalytic subunit;Calmodulin-dependent calcineurin A subunit alpha isoform;PPP3CA;CALNA, CNA;

Gene Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

UniProt

Q08209

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Calcineurin A, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cell membrane . Cell membrane, sarcolemma . Nucleus . Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. .

Sequence Similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Protein Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform