Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® (Dylight488 Conjugate)

About this product

Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1463-Dylight488

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Synonyms

Dihydrolipoyl dehydrogenase, mitochondrial;1.8.1.4;Dihydrolipoamide dehydrogenase;Glycine cleavage system L protein;DLD;GCSL, LAD, PHE3;

Gene Name

Dihydrolipoyl dehydrogenase, mitochondrial

UniProt

P09622

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Placenta.

Applications

IF,IHC,IHC-F,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse<br> Immunocytochemistry , 0.5-1μg/ml, Human, -<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br>

Subcellular Location

Mitochondrion matrix.

Sequence Similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Protein Name

Dihydrolipoyl dehydrogenase, mitochondrial