Anti-SYBL1/VAMP7 Antibody Picoband® (Dylight550 Conjugate)

About this product

Boster Bio Anti-SYBL1/VAMP7 Antibody Picoband® catalog # PB10017. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10017-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Vesicle-associated membrane protein 7 (VAMP-7), also known as SYBL1, is a protein that in humans is encoded by the VAMP7 gene. This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide- sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.

Synonyms

Vesicle-associated membrane protein 7;VAMP-7;Synaptobrevin-like protein 1;Tetanus-insensitive VAMP;Ti-VAMP;VAMP7;SYBL1;

Gene Name

Vesicle-associated membrane protein 7

UniProt

P51809

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human SYBL1 recombinant protein (Position: N24-T190). Human SYBL1 shares 97.6% and 95.2% amino acid (aa) sequence identity with mouse and rat SYBL1, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected in all tissues tested.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Rat<br>

Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type IV membrane protein . Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein . Late endosome membrane ; Single-pass type IV membrane protein . Lysosome membrane ; Single-pass type IV membrane protein . Endoplasmic reticulum membrane ; Single-pass type IV membrane protein . Cytoplasmic vesicle, phagosome membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney (By similarity). .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

Vesicle-associated membrane protein 7