Anti-SP1 Antibody Picoband®, Dylight550 Conjugate

About this product

Boster Bio Anti-SP1 Antibody Picoband® catalog # A00110-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00110-1-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Synonyms

Transcription factor Sp1; SP1; TSFP1

Gene Name

transcription factor

Gene ID

6667

UniProt

P08047

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human SP1 recombinant protein (Position: Q384-A603).

Clonality

Polyclonal

Tissue Specificity

Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Gaynor, R. B., Shieh, B.-H., Klisak, I., Sparkes, R. S., Lusis, A. J.Localization of the transcription factor SP1 gene to human chromosome 12q12-q13.2.Cytogenet. Cell Genet. 64: 210-212, 1993. 2. Matera, A. G., Ward, D. C.Localization of the human Sp1 transcription factor gene to 12q13 by fluorescence in situ hybridization.Genomics 17: 793-794, 1993. 3. Wimmer, E. A., Jackle, H., Pfeifle, C., Cohen, S. M.A Drosophila homologue of human Sp1 is a head-specific segmentation gene.Nature 366: 690-694, 1993.Wimmer, E. A., Jackle, H., Pfeifle, C., Cohen, S. M.A Drosophila homologue of human Sp1 is a head-specific segmentation gene.Nature 366: 690-694, 1993.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33275 MW

Observed Molecular Weight

90 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml<br> Immunocytochemistry/Immunofluorescence, 5μg/ml<br> Immunofluorescence, 5μg/ml<br> Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.

Protein Name

Programmed cell death 1 ligand 1