Anti-TIMM17A Antibody Picoband®, Dylight550 Conjugate

About this product

Boster Bio Anti-TIMM17A Antibody Picoband® catalog # A12168-1. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12168-1-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Mitochondrial import inner membrane translocase subunit Tim17-A is an enzyme that in humans is encoded by the TIMM17A gene. It is mapped to 1q32.1. TIM17 is a conserved suppressor of mtDNA instability, and overexpression of TIMM17A can prevent mtDNA loss in a human cellular model of mitochondrial disease.

Synonyms

Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a; TIMM17A; MIMT17; TIM17; TIM17A; TIMM17

Gene Name

translocase of inner mitochondrial membrane 17A

Gene ID

10440

UniProt

Q99595

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TIMM17A, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat TIMM17A, respectively.

Clonality

Polyclonal

Applications

Flow Cytometry,IF,IHC,IHC-F,ICC,WB

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

References & Citations

1. Bauer, M. F., Gempel, K., Reichert, A. S., Rappold, G. A., Lichtner, P., Gerbitz, K. D., Neupert, W., Brunner, M., Hofmann, S. Genetic and structural characterization of the human mitochondrial inner membrane translocase. J. Molec. Biol. 289: 69-82, 1999. 2. Bomer, U., Rassow, J., Zufall, N., Pfanner, N., Meijer, M., Maarse, A. C. The preprotein translocase of the inner mitochondrial membrane: evolutionary conservation of targeting and assembly of Tim17. J. Molec. Biol. 262: 389-395, 1996. 3. Iacovino, M., Granycome, C., Sembongi, H., Bokori-Brown, M., Butow, R. A., Holt, I. J., Bateman, J. M. The conserved translocase Tim17 prevents mitochondrial DNA loss. Hum. Molec. Genet. 18: 65-74, 2009.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

18 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml<br> Immunocytochemistry/Immunofluorescence, 2μg/ml<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells<br>

Subcellular Location

Mitochondrion inner membrane, Multi-pass membrane protein.