Anti-CENPI Antibody Picoband®, Dylight550 Conjugate

About this product

Boster Bio Anti-CENPI Antibody Picoband® catalog # A10263-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A10263-1-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Centromere protein I is a protein that in humans is encoded by the CENPI gene.

Synonyms

Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA

Gene Name

pre T cell antigen receptor alpha

Gene ID

2491

UniProt

Q92674

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CENPI recombinant protein (Position: K120-C604).

Clonality

Polyclonal

Tissue Specificity

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Liu, S.-T., Hittle, J. C., Jablonski, S. A., Campbell, M. S., Yoda, K., Yen, T. J. Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis. Nature Cell Biol. 4: 341-345, 2003. 2. Roberts, R. G., Kendall, E., Vetrie, D., Bobrow, M. Sequence and chromosomal location of a human homologue of LRPR1, an FSH primary response gene. Genomics 37: 122-124, 1996. 3. Slegtenhorst-Eegdeman, K. E., Post, M., Baarends, W. M., Themmen, A. P. N., Grootegoed, J. A. Regulation of gene expression in Sertoli cells by follicle-stimulating hormone (FSH): cloning and characterization of LRPR1, a primary response gene encoding a leucine-rich protein. Molec. Cell. Endocr. 108: 115-124, 1995.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

41409 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Protein NDRG3