Anti-Macrosialin CD68 Antibody Picoband®, Dylight550 Conjugate

About this product

<p class="text-grey mb-2"><strong>Superstar antibody:</strong> Boster’s anti mouse CD68 antibody (PA1518) is among its top 10 bestselling antibodies, and one of the most cited mouse/rat CD68 antibodies on the market. Because of this antibody’s high demand, we have subsequently developed <a href="/anti-cd68-rabbit-monoclonal-antibody-m00602-1-boster.html" target="_blank">a rabbit monoclonal anti CD68 antibody (M00602-1)</a> targetting a similar epitope.</p> <p class="text-grey mb-2"><strong>High specificity:</strong> PA1518 reacts with murine macrosialin/CD68, and has been validated with Western blotting and confirmed its stellar specificity. CD68 is a highly glycosylated protein, and its expected western blot molecular weight is between 80kDa to 110kDa, depending on glycosylation level. PA1518’s observed MW in WB is near 90-100kDa. Testing on negative control tissues showed no significant bands (images available on request) or staining. See more info in the positive and negative control design section. Based on immunogen sequence homology, this antibody is not expected to cross react with other proteins from the LAMP family, which complies with QC testing observations. </p> <p class="text-grey mb-2"><strong>Great for CD68 IHC:</strong> This antibody produces clean and specific immunobiological stains in both mouse and rat tissues. Our QC team has validated it on spleen and liver tissues of both mice and rats. Click product images for more details on experiment conditions.</p> <p class="text-grey mb-2"><strong>More about CD68:</strong> CD68 is a cell surface marker often used to identify macrophages and other cell types in the monocyte lineage. It is a transmembrane protein that binds to electins and selectins and plays a role in macrophage homing movement. Check out the <a href="/bosterbio-gene-info-cards/CD68" target="_blank">CD68 </a>biomarker page for more information on CD68 and view all CD68 antibodies, ELISA kits and proteins. </p> The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1518-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells. CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be ed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.

Synonyms

Macrosialin;CD68;Cd68;

Gene Name

Macrosialin

UniProt

P31996

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse CD68, different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in tissue macrophages and to a lesser extent in dendritic cells.

Applications

IF,IHC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Holness, C. L., Simmons, D. L.Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.Blood 81: 1607-1613, 1993. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R.The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13.Genomics 53: 248-250, 1998.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34818 MW

Observed Molecular Weight

90-100 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat<br> Immunofluorescence, 5μg/ml, Mouse<br> Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Mouse<br>

Subcellular Location

Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the LAMP family.

Protein Name

Macrosialin