Anti-mtTFA/TFAM Antibody Picoband®, Dylight550 Conjugate

About this product

Boster Bio Anti-mtTFA/TFAM Antibody catalog # PA1936. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1936-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Synonyms

Transcription factor A, mitochondrial;mtTFA;Mitochondrial transcription factor 1;MtTF1;Transcription factor 6;TCF-6;Transcription factor 6-like 2;TFAM;TCF6, TCF6L2;

Gene Name

Transcription factor A, mitochondrial

UniProt

Q00059

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human mtTFA.

Clonality

Polyclonal

Tissue Specificity

Widely expressed in different tissues.

Applications

IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Larsson, N.-G., Oldfors, A., Garman, J. D., Barsh, G. S., Clayton, D. A. Down-regulation of mitochondrial transcription factor A during spermatogenesis in humans. Hum. Molec. Genet. 6: 185-191, 1997. 2. Milatovich, A., Parisi, M. A., Poulton, J., Clayton, D. A., Francke, U. Sequences homologous to MTTF1, mitochondrial transcription factor 1, are located on human chromosomes 7 (7pter-cen), 10 and 11 (11cen-qter). (Abstract) Cytogenet. Cell Genet. 58: 1924 only, 1992. 3. Scott, A. F. Personal Communication. Baltimore, Md. 9/20/2007.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29097 MW

Observed Molecular Weight

24 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br>

Subcellular Location

Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.

Sequence Similarities

Belongs to the FAM10 family.

Protein Name

Transcription factor A, mitochondrial