Anti-SLC12A6 Antibody Picoband®, Dylight550 Conjugate

About this product

Boster Bio Anti-SLC12A6 Antibody catalog # PA2171. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2171-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Solute carrier family 12 (potassium/chloride transporters), member 6, also called SLC12A6 or KCC3, is a protein that in humans is encoded by the SLC12A6 gene. This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. By radiation hybrid and somatic cell hybrid analyses, this gene is mapped to 15q14. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy.

Synonyms

Solute carrier family 12 member 6;Electroneutral potassium-chloride cotransporter 3;K-Cl cotransporter 3;SLC12A6;KCC3;

Gene Name

Solute carrier family 12 member 6

UniProt

Q9UHW9

Host

Rabbit

Reactivity

Rat,Mouse,Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SLC12A6, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level). .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Howard, H. C., Mount, D. B., Rochefort, D., Byun, N., Dupre, N., Lu, J., Fan, X., Song, L., Riviere, J.-B., Prevost, C., Horst, J., Simonati, A., and 12 others. The K-Cl cotransporter KCC3 is mutant in a severe peripheral neuropathy associated with agenesis of the corpus callosum. Nature Genet. 32: 384-392, 2002. Note: Erratum: Nature Genet. 32: 681 only, 2002. 2. Salin-Cantegrel, A., Riviere, J.-B., Shekarabi, M., Rasheed, S., DaCal, S., Laganiere, J., Gaudet, R., Rochefort, D., Lesca, G., Gaspar, C., Dion, P. A., Lapointe, J.-Y., Rouleau, G. A.Transit defect of potassium-chloride co-transporter 3 is a major pathogenic mechanism in hereditary motor and sensory neuropathy with agenesis of the corpus callosum. J. Biol. Chem. 286: 28456-28465, 2011.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

127617 MW

Observed Molecular Weight

200 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat<br> Immunohistochemistry, 2-5 μg/ml, Human<br>

Subcellular Location

Basolateral cell membrane ; Multi-pass membrane protein .

Protein Name

Solute carrier family 12 member 6