Anti-Cathepsin B/CTSB Antibody Picoband®, Dylight550 Conjugate

About this product

Boster Bio Anti-Cathepsin B/CTSB Antibody Picoband® catalog # PB9046. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9046-Dylight550

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

<a href="https://www.bosterbio.com/anti-cathepsin-b-picoband-trade-antibody-pb9046-boster.html" style="color:#ea8d28">Cathepsin B</a> is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Synonyms

Cathepsin B;3.4.22.1;APP secretase;APPS;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;CTSB;CPSB;

Gene Name

Cathepsin B

Gene ID

1508

UniProt

P07858

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in all cell types so far analyzed.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Chan SJ, San Segundo B, McCormick MB, Steiner DF (October 1986). "Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs". Proc. Natl. Acad. Sci. U.S.A. 83 (20): 7721–5. 2. Cao L, Taggart RT, Berquin IM, Moin K, Fong D, Sloane BF (February 1994). "Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene". Gene 139 (2): 163–9. 3. Wang, X.; Chan, S. J.; Eddy, R. L.; Byers, M. G.; Fukushima, Y.; Henry, W. M.; Haley, L. L.; Steiner, D. F.; Shows, T. B. : Chromosome assignment of cathepsin B (CTSB) to 8p22 and cathepsin H (CTSH) to 15q24-q25. (Abstract) Cytogenet. Cell Genet. 46: 710-711, 1987. 4. Mueller-Steiner S, Zhou Y, Arai H, Roberson ED, Sun B, Chen J, Wang X, Yu G, Esposito L, Mucke L, Gan L (September 2006). "Antiamyloidogenic and neuroprotective functions of cathepsin B: implications for Alzheimer's disease". Neuron 51 (6): 703–14.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37822 MW

Observed Molecular Weight

33 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sequence Similarities

Belongs to the peptidase C1 family.

Protein Name

Cathepsin B