Anti-Grp75/HSPA9 Antibody Picoband®, Dylight594 Conjugate

About this product

Boster Bio Anti-Grp75/HSPA9 Antibody catalog # PA1789. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1789-Dylight594

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

HSPA9 (heat shock 70kDa protein 9 (mortalin)),also known as GRP75, mot-2, mthsp75, PBP74, HSPA9B, MORTALIN or MORTALIN, PERINUCLEAR, is a highly conserved member of the HSP70 family of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome. The HSPA9 gene is mapped to chromosome 5q31.2 based on an alignment of the HSPA9 sequence with the genomic sequence. Knockdown of HSPA9 in erythroid cultures was associated with an increased number of cells in the G0/G1 phase of the cell cycle and accelerated apoptosis. Knockdown of Hspa9 in mouse bone marrow cells, followed by transplantation into wildtype recipients, also resulted in loss of erythroid cell number. Haploinsufficiency for HSPA9 may contribute to abnormal hematopoiesis in myelodysplastic syndromes. This protein plays a role in the control of cell proliferation.

Synonyms

Stress-70 protein, mitochondrial;75 kDa glucose-regulated protein;GRP-75;Heat shock 70 kDa protein 9;Mortalin;MOT;Peptide-binding protein 74;PBP74;HSPA9;GRP75, HSPA9B, mt-HSP70;

Gene Name

Stress-70 protein, mitochondrial

UniProt

P38646

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Grp75, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Applications

IHC,IHC-F,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

73680 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat<br> Immunocytochemistry , 0.5-1μg/ml, Human<br>

Subcellular Location

Mitochondrion . Nucleus, nucleolus .

Sequence Similarities

Belongs to the heat shock protein 70 family.

Protein Name

Stress-70 protein, mitochondrial