Anti-ABCG1 Antibody Picoband®, Dylight594 Conjugate

About this product

Boster Bio Anti-ABCG1 Antibody catalog # PA1868. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1868-Dylight594

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

ABCG1 (Atp-binding cassette, subfamily g, member 1) also known as ABC8, ABC TRANSPORTER 8 or WHITE, DROSOPHILA, HOMOLOG OF, is a protein that in humans is encoded by the ABCG1 gene. ABCG1 is a regulator of macrophage cholesterol and phospholipid transport. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters.The ABCG1 gene is mapped on 21q22.3. This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. ABCG1 is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Inhibition of ABCG1 protein expression using an antisense strategy resulted in reduced HDL3-dependent efflux of cholesterol and choline-phospholipids. In macrophages, silencing of GPS2 by RNA interference reduced ABCG1 expression and diminished ABCG1-mediated cholesterol efflux.

Synonyms

ATP-binding cassette sub-family G member 1;ATP-binding cassette transporter 8;White protein homolog;ABCG1;ABC8, WHT1;

Gene Name

ATP-binding cassette sub-family G member 1

UniProt

P45844

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ABCG1, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Chen, H., Rossier, C., Lalioti, M. D., Lynn, A., Chakravarti, A., Perrin, G., Antonarakis, S. E. Cloning of the cDNA for a human homologue of the Drosophila white gene and mapping to chromosome 21q22.3. Am. J. Hum. Genet. 59: 66-75, 1996. 2. Jakobsson, T., Venteclef, N., Toresson, G., Damdimopoulos, A. E., Ehrlund, A., Lou, X., Sanyal, S., Steffensen, K. R., Gustafsson, J.-A., Treuter, E. GPS2 is required for cholesterol efflux by triggering histone demethylation, LXR recruitment, and coregulator assembly at the ABCG1 locus. Molec. Cell 34: 510-518, 2009. 3. Lorkowski, S., Rust, S., Engel, T., Jung, E., Tegelkamp, K., Galinski, E. A., Assmann, G., Cullen, P. Genomic sequence and structure of the human ABCG1(ABC8) gene. Biochem. Biophys. Res. Commun. 280: 121-131, 2001.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

75592 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Protein Name

ATP-binding cassette sub-family G member 1