Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® (PE Conjugate)

About this product

Boster Bio Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® catalog # PB9114. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9114-PE

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. It is mapped to 1q44. The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, it has been showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Synonyms

NACHT, LRR and PYD domains-containing protein 3;Angiotensin/vasopressin receptor AII/AVP-like;Caterpiller protein 1.1;CLR1.1;Cold autoinflammatory syndrome 1 protein;Cryopyrin;PYRIN-containing APAF1-like protein 1;NLRP3;C1orf7, CIAS1, NALP3, PYPAF1;

Gene Name

NACHT, LRR and PYD domains-containing protein 3

UniProt

Q96P20

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

Clonality

Polyclonal

Tissue Specificity

Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cytoplasm .

Sequence Similarities

Belongs to the NLRP family.

Protein Name

NACHT, LRR and PYD domains-containing protein 3