Anti-SLC12A1 Antibody Picoband® (PE Conjugate)

About this product

Boster Bio Anti-SLC12A1 Antibody Picoband® catalog # PB9392. Tested in IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9392-PE

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Solute carrier family 12 (sodium/potassium/chloride transporters), member 1, also called NKCC2 is specifically found in cells of the thick ascending limb of the loop of Henle in nephrons, the basic functional units of the kidney. This gene is mapped to 15q21.1. This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. This gene plays a vital role in the regulation of ionic balance and cell volume.

Synonyms

Solute carrier family 12 member 1;Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 2;Kidney-specific Na-K-Cl symporter;SLC12A1;NKCC2;

Gene Name

Solute carrier family 12 member 1

UniProt

Q13621

Host

Rabbit

Reactivity

Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Kidney specific.

Applications

IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Monkey, Mouse, Rat <br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat<br> Immunofluorescence, 5 μg/ml, Mouse, Rat <br>

Subcellular Location

Membrane; Multi-pass membrane protein.

Sequence Similarities

Contains 1 PID domain.

Protein Name

Solute carrier family 12 member 1