Anti-MAPK6 Antibody Picoband® (PE Conjugate)

About this product

Boster Bio Anti-MAPK6 Antibody Picoband® catalog # PB9666. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9666-PE

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitogen-activated protein kinase 6 is an enzyme that in humans is encoded by the MAPK6 gene. It is mapped to 15q21. The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblastsupon treatment with serum or phorbol esters.

Synonyms

Mitogen-activated protein kinase 6;MAP kinase 6;MAPK 6;2.7.11.24;Extracellular signal-regulated kinase 3;ERK-3;MAP kinase isoform p97;p97-MAPK;MAPK6;ERK3, PRKM6;

Gene Name

Mitogen-activated protein kinase 6

UniProt

Q16659

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human MAPK6 recombinant protein (Position: E520-N721). Human MAPK6 shares 86.2% and 82.8% amino acid (aa) sequence identity with mouse and rat MAPK6, respectively.

Clonality

Polyclonal

Tissue Specificity

Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity). .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat <br>Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Cytoplasm . Nucleus . Translocates to the cytoplasm following interaction with MAPKAPK5. .

Protein Name

Mitogen-activated protein kinase 6